Application
Immunohistochemical analysis of paraffin-embedded human gastric, using PKC iota antibody at 1:100 dilution.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKC iota antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Sample(30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500| Product Name | PKC iota antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PKC iota (protein kinase C, iota)This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKC iota. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, BovineMouse (97%), Rat (97%), Zebrafish (89%), Xenopus laevis (91%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DXS1179E antibody, MGC26534 antibody, PKCI antibody, nPKC-iota antibody, PRKCI antibody, atypical protein kinase C-lambda/iota antibody, protein kinase C iota type antibody, PRKC-lambda/iota antibody, aPKC-lambda/iota antibody, "protein kinase C, iota anti |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) |
| Cellular Localization | Cytoplasm , Membrane , Endosome , Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded human gastric, using PKC iota antibody at 1:100 dilution.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PKC iota antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Sample(30 ug whole cell lysate) A:293T 7.5% SDS PAGE diluted at 1:500| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
