Application
PKC zeta antibody detects PKC zeta protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PKC zeta antibody dilution: 1:500.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta antibody diluted at 1:1000.| Product Name | PKC zeta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PKC zeta (protein kinase C, zeta)Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PKC zeta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Pig, Rabbit, BovineMouse (99%), Rat (99%), Zebrafish (91%), Pig (99%), Rabbit (99%), Bovine (99%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PKC-ZETA antibody, PKC2 antibody, PRKCZ antibody, nPKC-zeta antibody, protein kinase C zeta type antibody, "protein kinase C, zeta antibody" |
|---|---|
| Target | PKC zeta |
| Molecular Weight(MW) | 68 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Endosome |
Application
PKC zeta antibody detects PKC zeta protein at cytoplasm on SW480 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded SW480 xenograft. PKC zeta antibody dilution: 1:500.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PKC zeta antibody diluted at 1:1000.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
