Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000Application
PLA2G4F antibody [N3C2], Internal detects PLA2G4F protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLA2G4F protein stained by PLA2G4F antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PLA2G4F antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLA2G4F (phospholipase A2, group IVF)Calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Has higher enzyme activity for phosphatidylethanolamine than phosphatidylcholine. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PLA2G4F. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp666G192 antibody, DKFZp781B229 antibody, PLA2G4FZ antibody, PLA2G4F antibody, cytosolic phospholipase A2 zeta antibody, cPLA2-zeta antibody, "phospholipase A2, group IVF antibody" |
|---|---|
| Molecular Weight(MW) | 95 kDa (note) (2) |
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:1000
Application
PLA2G4F antibody [N3C2], Internal detects PLA2G4F protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLA2G4F protein stained by PLA2G4F antibody [N3C2], Internal diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , A431 , Jurkat , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
