Application
Immunofluorescence analysis of methanol-fixed HeLa, using PLC delta 1 antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PLCD1 antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PLCD1 antibody [C1C3] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCD1 (phospholipase C, delta 1)Phosphoinositide-specific phospholipase C (PLC) acts as a signal transducer that generates 2 second messengers, diacylglycerol and inositol 1,4,5-trisphosphate, by hydrolyzing inositol phospholipids. PLC comprises a diverse family of enzymes that differ in structure and tissue distribution (Berridge, 1993 [PubMed 8381210]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PLCD1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (86%), Rat (86%), Pig (92%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PLCD1 antibody, "1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-1 antibody", PLC-delta-1 antibody, phospholipase C-III antibody, phosphoinositide phospholipase C-delta-1 antibody, "phospholipase C, delta 1 antibody" |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (3) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using PLC delta 1 antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using PLCD1 antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: HeLa 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
