Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PLCD3 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Product Name | PLCD3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCD3 (phospholipase C, delta 3)This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PLCD3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (84%), Rat (82%), Bovine (85%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC71172 antibody, PLCD3 antibody, PLC delta3 antibody, "1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3 antibody", PLC-delta-3 antibody, phosphoinositide phospholipase C-delta-3 antibody, phospholipase C-delta-3 antibody, "phospholipase |
|---|---|
| Molecular Weight(MW) | 89 kDa (note) (2) |
| Cellular Localization | Membrane; Peripheral membrane protein , Cytoplasm |
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using PLCD3 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:1000| Positive Control | HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
