Application
Immunofluorescence staining of methanol-fixed Hela cells using PLC gamma 2 (Phospho-Tyr753) Antibody .Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLC gamma 2 (Phospho-Tyr753) Antibody .Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody| Product Name | PLCG2 (phospho Tyr753) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PLCG2 (Phospho Tyr753)The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling. |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 753 (S-L-Y(p)-D-V) derived from Human PLC gamma2. |
| Modification | p-Tyr753 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PLCG2, phospholipase C-IV, phospholipase C gamma 2, phospholipase C, gamma 2 (phosphatidylyinositol-specific), phospholipase C-gamma-2, phosphoinositide phospholipase C-gamma-2, PLC-IV, PLC-gamma-2, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase |
|---|---|
| Target | PLC gamma2 (Phospho Tyr753) |
| Molecular Weight(MW) | 150 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using PLC gamma 2 (Phospho-Tyr753) Antibody .
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLC gamma 2 (Phospho-Tyr753) Antibody .
Application
Western blot analysis of extracts from HepG2 cells untreated or treated with EGF using PLCg2(Phospho-Tyr753) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
