Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000Application
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PLRG1 antibody [C3], C-term dilution: 1:500.| Product Name | PLRG1 antibody [C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PLRG1 (pleiotropic regulator 1 (PRL1 homolog, Arabidopsis))Necessary for spliceosome assembly and for pre-mRNA splicing. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PLRG1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chimpanzee, BovineRat (100%), Xenopus laevis (100%), Pig (100%), Chimpanzee (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC110980 antibody, PRL1 antibody, PRP46 antibody, PRPF46 antibody, PLRG1 antibody, "pleiotropic regulator 1 (PRL1 homolog, Arabidopsis) antibody", pleiotropic regulator 1 antibody |
|---|---|
| Target | PLRG1 |
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Nucleus speckle |
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000
Application
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus by immunofluorescent analysis. Sample: H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PLRG1 protein stained by PLRG1 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
PLRG1 antibody [C3], C-term detects PLRG1 protein at nucleus on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PLRG1 antibody [C3], C-term dilution: 1:500.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
