Application
Immunoprecipitation of PML protein from A549 whole cell extracts using 5 μg of PML antibody [N1N2], N-term . Western blot analysis was performed using PML antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:5000| Product Name | PML antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PML (promyelocytic leukemia)The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL). Extensive alternative splicing of this gene results in several variations of the protein's central and C-terminal regions; all variants encode the same N-terminus. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PML. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, Chimpanzee, BovineRhesus Monkey (98%), Chimpanzee (100%), Bovine (84%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MYL antibody PP8675 antibody RNF71 antibody TRIM19 antibody PML antibody RING finger protein 71 antibody "promyelocytic leukemia inducer of antibody" tripartite motif protein TRIM19 antibody protein PML antibody promyelocytic leukemia protein ant |
|---|---|
| Molecular Weight(MW) | 98 kDa (note) (9) |
Application
Immunoprecipitation of PML protein from A549 whole cell extracts using 5 μg of PML antibody [N1N2], N-term . Western blot analysis was performed using PML antibody [N1N2], N-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:5000| Positive Control | A549 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
