Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PNPase antibody at 1:250 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Product Name | PNPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PNPase (polyribonucleotide nucleotidyltransferase 1)PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human PNPase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, BovineRat (90%), Pig (90%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp762K1914 antibody OLD35 antibody PNPASE antibody old-35 antibody PNPT1 antibody 3'-5' RNA exonuclease OLD35 antibody polynucleotide phosphorylase-like protein antibody PNPase 1 antibody PNPase old-35 antibody "polyribonucleotide nucleotidyltr |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PNPase antibody at 1:250 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: JurKat 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , Jurkat , Raji , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
