Application
Immunoprecipitation of PNPase protein from HeLa whole cell extracts using 5 μg of PNPase antibody . Western blot analysis was performed using PNPase antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
PNPase antibody detects PNPase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody diluted by 1:2500.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. PNPase antibody dilution: 1:250.| Product Name | PNPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PNPase (polyribonucleotide nucleotidyltransferase 1)PNPT1 is a subunit of the exosome complex, which is involved in 3-prime-to-5-prime exoribonuclease activity for RNA processing and degradation (Raijmakers et al., 2002 [PubMed 12419256]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PNPase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, BovineMouse (92%), Rat (92%), Pig (92%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp762K1914 antibody, OLD35 antibody, PNPASE antibody, old-35 antibody, PNPT1 antibody, 3'-5' RNA exonuclease OLD35 antibody, polynucleotide phosphorylase-like protein antibody, PNPase 1 antibody, PNPase old-35 antibody, "polyribonucleotide nucleotidyltr |
|---|---|
| Molecular Weight(MW) | 86 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunoprecipitation of PNPase protein from HeLa whole cell extracts using 5 μg of PNPase antibody . Western blot analysis was performed using PNPase antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
PNPase antibody detects PNPase protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PNPase antibody diluted by 1:2500.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PNPase antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
PNPase antibody detects PNPase protein at mitochondria on human normal colon by immunohistochemical analysis. Sample: Paraffin-embedded human normal colon. PNPase antibody dilution: 1:250.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
