Application
POLR2D antibody detects POLR2D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: POLR2D protein stained by POLR2D antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
POLR2D antibody detects POLR2D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with POLR2D antibody diluted by 1:1000.| Product Name | POLR2D antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POLR2D (polymerase (RNA) II (DNA directed) polypeptide D)This gene encodes the fourth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. In yeast, this polymerase subunit is associated with the polymerase under suboptimal growth conditions and may have a stress protective role. A sequence for a ribosomal pseudogene is contained within the 3' untranslated region of the transcript from this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POLR2D. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, BovineMouse (100%), Rat (100%), Zebrafish (95%), Xenopus laevis (93%), Chicken (96%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HSRPB4 HSRBP4 polymerase (RNA) II (DNA directed) polypeptide D RPB16 RBP4 |
|---|---|
| Target | POLR2D |
| Molecular Weight(MW) | 16 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
POLR2D antibody detects POLR2D protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde/PBS for 15 min. Green: POLR2D protein stained by POLR2D antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
POLR2D antibody detects POLR2D protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with POLR2D antibody diluted by 1:1000.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
