Application
POMGNT1 antibody detects POMGNT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. POMGNT1 antibody diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed Hep3B, using POMGNT1 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using POMGNT1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | POMGNT1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to POMGNT1 (protein O-linked mannose beta1,2-N-acetylglucosaminyltransferase)The protein encoded by this gene is a type II transmembrane protein that resides in the golgi. It participates in O-mannosyl glycosylation, and is specific for alpha linked terminal mannose. Mutations in this gene are associated with muscle-eye-brain (MEB) disease. Alternatively spliced transcript variants have been found for this gene. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human POMGNT1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, BovineRat (99%), Xenopus laevis (84%), Pig (98%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp761B182 antibody FLJ20277 antibody GNTI.2 antibody MDDGA3 antibody MDDGB3 antibody MDDGC3 antibody MEB antibody MGAT1.2 antibody POMGNT1 antibody "UDP-GlcNAc:alpha-D-mannoside beta-1 2-N-acetylglucosaminyltransferase I.2 antibody" "protein O |
|---|---|
| Molecular Weight(MW) | 75 kDa (note) (4) |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein |
Application
POMGNT1 antibody detects POMGNT1 protein at cytoplasm in mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. POMGNT1 antibody diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed Hep3B, using POMGNT1 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using POMGNT1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
