Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 B: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | PP1C gamma antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PP1C gamma (protein phosphatase 1, catalytic subunit, gamma isozyme)Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PP1C gamma. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, BovineRat (100%), Xenopus laevis (100%), Dog (99%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PP1gamma antibody PPP1G antibody PPP1CC antibody protein phosphatase 1C catalytic subunit antibody serine/threonine-protein phosphatase PP1-gamma catalytic subunit antibody serine/threonine phosphatase 1 gamma antibody "protein phosphatase 1 catalyt |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
Application
Sample (30 µg of whole cell lysate) A: NIH-3T3 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP1 gamma antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1CC antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 B: Molt-4 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
