Application
PP2A alpha antibody detects PP2A alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody . Western blot analysis was performed using PP2A alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | PP2A alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PP2A alpha (protein phosphatase 2, catalytic subunit, alpha isozyme)This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PP2A alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Chicken, BovineMouse (100%), Rat (100%), Zebrafish (100%), Xenopus laevis (100%), Dog (100%), Pig (100%), Rabbit (100%), Chicken (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PP2CA PPP2CA PP2Calpha PP2Ac protein phosphatase 2 catalytic subunit alpha isozyme RP-C |
|---|---|
| Target | PP2A alpha |
| Molecular Weight(MW) | 36 kDa (note) |
| Cellular Localization | Cytoplasm , Nucleus , Centromere , Spindle |
Application
PP2A alpha antibody detects PP2A alpha protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PP2A alpha antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of PP2A alpha protein from 293T whole cell extracts using 5 μg of PP2A alpha antibody . Western blot analysis was performed using PP2A alpha antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
