Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2A beta antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:500| Product Name | PP2A beta antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PP2A beta (protein phosphatase 2, catalytic subunit, beta isozyme)This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PP2A beta. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Rabbit, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (100%), Xenopus laevis (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PP2Abeta antibody PP2CB antibody PPP2CB antibody "protein phosphatase 2 (formerly 2A) catalytic subunit beta isoform antibody" "protein phosphatase 2 catalytic subunit beta isoform antibody" "protein phosphatase 2A catalytic subunit beta isoform |
|---|---|
| Molecular Weight(MW) | 36 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , Centromere , Spindle |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2A beta antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 12% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
