Application
WB analysis of PARP alpha expression in Jurkat (1,2), rat liver (3), rat kidney (4).| Product Name | PPAR alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to PPAR alphaPeroxisome proliferators are nongenotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed Peroxisome Proliferator Activated Receptors (PPARs). Nuclear hormone receptors are ligand dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR alpha, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex. |
| Immunogen | Synthetic peptide surrounding amino acid 25 of human PPARa. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500~1:2000 |
|
| Species Reactivity | HumanMousePigRatSheep |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PPAR hPPAR MGC2452 PPARA NR1C1 Peroxisome Proliferator Activated Receptor alpha PPAR alpha MGC2237 PPARalpha |
|---|---|
| Target | PPAR alpha |
| Cellular Localization | Nuclear |
Application
WB analysis of PARP alpha expression in Jurkat (1,2), rat liver (3), rat kidney (4).| Positive Control | Rat liver. Baboon myometrium single band at ~52 kDa. K562 cells three bands: 52 kDa band and ~60 kDa band have the similar density and a much weaker band at ~45-50 kDa. |
|---|---|
| Application Notes | WB:1:500~1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide Constituents: 50% Glycerol, 1% BSA, PBS |
