Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using PPM1B antibody at 1:500 dilution.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2C beta antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 10% SDS PAGE diluted at 1:1000| Product Name | PPM1B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPM1B (protein phosphatase, Mg2+/Mn2+ dependent, 1B)The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPM1B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (94%), Rat (96%), Xenopus laevis (89%), Chicken (96%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC21657 antibody PP2C-beta-X antibody PP2CB antibody PP2CBETA antibody PPC2BETAX antibody PPM1B antibody "protein phosphatase 1B (formerly 2C) magnesium-dependent beta isoform antibody" PP2C-beta antibody protein phosphatase 1B antibody protein |
|---|---|
| Molecular Weight(MW) | 53 kDa (note) (4) |
Application
Immunohistochemical analysis of paraffin-embedded PC9 xenograft, using PPM1B antibody at 1:500 dilution.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PP2C beta antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 D: Hela 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
