Application
WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A antibody at 1:100 dilution.| Product Name | PPP1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP1A (protein phosphatase 1, catalytic subunit, alpha isozyme)The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Xenopus laevis, Dog, Pig, Rabbit, BovineMouse (99%), Xenopus laevis (97%), Dog (100%), Pig (100%), Rabbit (97%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC15877 antibody MGC1674 antibody PP-1A antibody PP1alpha antibody PPP1A antibody PPP1CA antibody serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit antibody "protein phosphatase 1 catalytic subunit alpha isoform antibody" serine |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
WB to detect PP1 alpha from U2OS cells treated with control siRNA or PP1 alpha siRNA, using at 1:1000 dilution. Nuclear matrix protein p84 is a loading control, blotted with p84 antibody (clone 5E10) at 1:5000 dilution. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPP1A antibody detects PPP1A protein by western blot analysis. Rat tissue extracts (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPP1A antibody detects PPP1A protein by western blot analysis. Whole cell extracts (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with PPP1A antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using PPP1A antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2500.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using PPP1A antibody at 1:100 dilution.| Positive Control | 293T , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
