Application
PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PPP1CB antibody dilution: 1:500.Application
PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PPP1CB antibody dilution: 1:500.Application
Sample (30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: PPP1CB protein stained by PPP1CB antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PPP1CB antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP1CB (protein phosphatase 1, catalytic subunit, beta isozyme)The protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Mouse studies suggest that PP1 functions as a suppressor of learning and memory. Two alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP1CB. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Dog, Pig, Rabbit, Chicken, Rhesus Monkey, BovineRat (100%), Zebrafish (99%), Xenopus laevis (100%), Dog (99%), Pig (100%), Rabbit (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC3672 antibody PP-1B antibody PP1beta antibody PPP1CD antibody PPP1CB antibody protein phosphatase 1-beta antibody protein phosphatase 1-delta antibody "protein phosphatase 1 catalytic subunit delta isoform antibody" serine/threonine protein ph |
|---|---|
| Molecular Weight(MW) | 37 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. PPP1CB antibody dilution: 1:500.
Application
PPP1CB antibody detects PPP1CB protein at nucleus and cytosol on mouse hind brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse hind brain. PPP1CB antibody dilution: 1:500.
Application
Sample (30 µg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: HepG2 F: MOLT4 G: Raji 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (50 µg of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPP1CB antibody detects PPP1CB protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min. Green: PPP1CB protein stained by PPP1CB antibody diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
