Application
Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).| Product Name | 4E BP1 (phospho Thr45) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | BP-1, 4EBP1, 4E-BP1, PHAS-I, STK14A, EIF4EBP1, PHAS-1 |
| Product description | 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1) Gingras AC, et al. (1998) Genes Dev 12 (4): 502-513. Brugarolas J, et al. (2004) Genes Dev 18 (23): 2893-2904. Kumar V, et al. (2000) EMBO J 19 (5): 1087-1097. Moody CA, et al. (2005) J Virol 79 (9): 5499-5506. Burnett PE, et al. (1998) Proc Natl Acad Sci U S A 95 (4): 1432-1437. |
| Immunogen | 4E-BP1 (Phospho-Thr45) antibody was raised against a peptide sequence around phosphorylation site of threonine 45 (S-T-T (p) -P-G) derived from Human 4E-BP1. |
| Modification | p-Thr45 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Alternative Names | BP-1 4EBP1 4E-BP1 PHAS-I STK14A EIF4EBP1 PHAS-1 |
| Molecular Weight(MW) | 18 kDa |
| Tissue Specificity | This antibody detects endogenous level of 4E-BP1 only when phosphorylated at Threonine 45. |
| Entrez Gene | 1978 |
|---|---|
| Protein Accession | NP_004086.1 |
Application
Western blot analysis of lysed extracts from HeLa cells using 4E-BP1 (Phospho-Thr45).
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 (Phospho-Thr45).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
