Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | 4E-BP1 (phospho Thr70) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 4EBP1, P/OKCL.6, P/OKCL.6, PHAS-I |
| Product description | 4E-BP1 encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.1) Pause A., Nature 371:762-767(1994). |
| Immunogen | 4E-BP1 (phospho Thr70) antibody was raised against a peptide sequence around phosphorylation site of threonine70 (T-K-T (p)-P-P) derived from Human 4E-BP1. |
| Modification | p-Thr70 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4EBP1 |
|---|---|
| Alternative Names | 4EBP1 P/OKCL.6 P/OKCL.6 PHAS-I |
| Molecular Weight(MW) | 12 kDa |
| Tissue Specificity | The antibody detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. |
| Entrez Gene | 1978 |
|---|---|
| Protein Accession | NP_004086.1 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 (Phospho-Thr70) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
