Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPP2R3A antibody at 1:250 dilution.Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: U87-MG 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | PPP2R3A antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP2R3A (protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha)Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human PPP2R3A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (90%), Rat (91%), Rhesus Monkey (97%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | PPP2R3 antibody PR130 antibody PR72 antibody PPP2R3A antibody "protein phosphatase 2 (formerly 2A) regulatory subunit B'' (PR 72) alpha isoform and (PR 130) beta isoform antibody" serine/threonine-protein phosphatase 2A regulatory subunit B'' subun |
|---|---|
| Molecular Weight(MW) | 130 kDa (note) (4) |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PPP2R3A antibody at 1:250 dilution.
Application
Sample (30 µg of whole cell lysate) A: NT2D1 B: U87-MG 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
PPP2R3A antibody [C2C3], C-term detects PPP2R3A protein at cytoplasm by immunofluorescent analysis. Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | NT2D1 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
