Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.| Product Name | A1CF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A1CF, ACF, ACF64, ACF65, APOBEC1CF, ASP, MGC163391, RP11-564C4.2 |
| Product description | Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. A1CF has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined.Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined.1) Blanc, V., (2005) Mol. Cell. Biol. 25 (16), 7260-7269. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human A1CF. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| A1CF antibody can be used for detection of A1CF by ELISA at 1:1562500. A1CF antibody can be used for detection of A1CF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | A1CF |
|---|---|
| Alternative Names | A1CF ACF ACF64 ACF65 APOBEC1CF ASP MGC163391 RP11-564C4.2 |
| Molecular Weight(MW) | 65 kDa |
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in WB on Hum. Adult Placenta at 1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | A1CF antibody can be used for detection of A1CF by ELISA at 1:1562500. A1CF antibody can be used for detection of A1CF by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store A1CF antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
