Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 7.5% SDS PAGE diluted at 1:5000| Product Name | PPP3CC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to PPP3CC (protein phosphatase 3 (formerly 2B), catalytic subunit, gamma isoform)Calmodulin-dependent protein phosphatase, calcineurin, is involved in a wide range of biologic activities, acting as a Ca(2+)-dependent modifier of phosphorylation status. In testis, the motility of the sperm is thought to be controlled by cAMP-dependent phosphorylation and a unique form of calcineurin appears to be associated with the flagellum. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18 kD, respectively. At least 3 genes, calcineurin A-alpha (CALNA1; MIM 114105), calcineurin A-beta (CALNA2; MIM 114106), and calcineurin A-gamma (CALNA3), have been cloned for the catalytic subunit. These genes have been identified in humans, mice, and rats, and are highly conserved between species (90 to 95% amino acid identity).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP3CC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (93%), Rat (93%), Bovine (97%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CALNA3 antibody CNA3 antibody PP2Bgamma antibody PPP3CC antibody "calcineurin testis-specific catalytic subunit antibody" calmodulin-dependent calcineurin A subunit gamma isoform antibody "protein phosphatase 3 (formerly 2B) catalytic subunit gamm |
|---|---|
| Target | PPP3CC |
| Molecular Weight(MW) | 58 kDa (note) (4) |
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 7.5% SDS PAGE diluted at 1:5000| Positive Control | NT2D1 , IMR32 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
