Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:500Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:500Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPP4C antibody at 1:500 dilution.| Product Name | PPP4C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP4C (protein phosphatase 4, catalytic subunit)Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP4C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Rabbit, Rhesus Monkey, BovineRat (99%), Xenopus laevis (99%), Rabbit (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | PP4 antibody PP4C antibody PPH3 antibody PPP4 antibody PPX antibody PPP4C antibody serine/threonine-protein phosphatase 4 catalytic subunit antibody PP-X antibody "protein phosphatase X catalytic subunit antibody" "protein phosphatase 4 (formerly |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (4) |
| Cellular Localization | Cytoplasm , Nucleus , Centrosome |
Application
Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 10% SDS PAGE diluted at 1:500
Application
Sample (30 ug of whole cell lysate) A: Hep G2 10% SDS PAGE diluted at 1:500
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPP4C antibody at 1:500 dilution.| Positive Control | HepG2 , NIH-3T3 , JC , BCL-1 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
