Application
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 μg/mL.Application
Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 μg/mL.| Product Name | Aak1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aak1 Antibody: KIAA1048, AP2-associated protein kinase 1, Adaptor-associated kinase 1 |
| Product description | Aak1 Antibody: AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.1) Connor SD and Schmid SL. Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis. J. Cell Biol.2002; 156:921-9. |
| Immunogen | Aak1 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1. The immunogen is located within amino acids 190 - 240 of Aak1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AAK1 |
|---|---|
| Alternative Names | Aak1 Antibody: KIAA1048 AP2-associated protein kinase 1 Adaptor-associated kinase 1 |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Aak1 in A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 μg/mL.
Application
Immunocytochemistry of Aak1 in A-20 cells with Aak1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1466 - Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | Aak1 antibody can be used for detection of Aak1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aak1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Aak1 Antibody is supplied in PBS containing 0.02% sodium azide. |
