Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: PPP4R2 transfected 293T lysate 10% SDS PAGE diluted at 1:5000| Product Name | PPP4R2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PPP4R2 (serine/threonine-protein phosphatase 4 regulatory subunit 2)Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PPP4R2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, ChickenMouse (80%), Xenopus laevis (80%), Chicken (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC131930 antibody PP4R2 antibody PPP4R2 antibody serine/threonine-protein phosphatase 4 regulatory subunit 2 antibody "protein phosphatase 4 regulatory subunit 2 antibody" |
|---|---|
| Molecular Weight(MW) | 47 kDa (note) (2) |
| Cellular Localization | Centrosome |
Application
Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: PPP4R2 transfected 293T lysate 10% SDS PAGE diluted at 1:5000| Positive Control | A431 , Jurkat , PPP4R2 transfected 293T lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
