Application
ABCG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Confocal immunofluorescent analysis of ABCG1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).Application
Western blot analysis of ABCG1 antibody in mouse spleen tissue lysates (35ug/lane)Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ABCG1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP-binding cassette sub-family G member 1, ATP-binding cassette transporter 8, White protein homolog, ABCG1, ABC8, WHT1 |
| Product description | ABCG1 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. It is involved in macrophage cholesterol and phospholipids transport, and may regulate cellular lipid homeostasis in other cell types.1) Furuyama,S., J. Atheroscler. Thromb. 16 (3), 194-200 (2009) |
| Immunogen | This ABCG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 168-195 amino acids from the N-terminal region of human ABCG1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | ABCG1 |
|---|---|
| Alternative Names | ATP-binding cassette sub-family G member 1 ATP-binding cassette transporter 8 White protein homolog ABCG1 ABC8 WHT1 |
| Molecular Weight(MW) | 76 kDa |
Application
ABCG1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Confocal immunofluorescent analysis of ABCG1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Application
Western blot analysis of ABCG1 antibody in mouse spleen tissue lysates (35ug/lane)
Application
Flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IHC-P starting dilution is: 1:50~100 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
