Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Immunofluorescence analysis of ABI1 Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Application
Western blot analysis of ABI1 Antibody in MCF-7, CEM, Jurkat cell line lysates (35ug/lane)| Product Name | ABI1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Abl interactor 1, Abelson interactor 1, Abi-1, Abl-binding protein 4, AblBP4, Eps8 SH3 domain-binding protein, Eps8-binding protein, Nap1-binding protein, Nap1BP, Spectrin SH3 domain-binding protein 1, e3B1, ABI1, SSH3BP1 |
| Product description | ABI1 has been found to form a complex with EPS8 and SOS1, and is thought to be involved in the transduction of signals from Ras to Rac. In addition, this protein may play a role in the regulation of EGF-induced Erk pathway activation as well as cytoskeletal reorganization and EGFR signaling.1) Wang,C., et.al., Mol. Cancer Res.(10), 1031-1039 (2007) |
| Immunogen | This ABI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human ABI1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABI1 |
|---|---|
| Alternative Names | Abl interactor 1 Abelson interactor 1 Abi-1 Abl-binding protein 4 AblBP4 Eps8 SH3 domain-binding protein Eps8-binding protein Nap1-binding protein Nap1BP Spectrin SH3 domain-binding protein 1 e3B1 ABI1 SSH3BP1 |
| Molecular Weight(MW) | 55 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with ABI1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometric analysis of SK-Br-3 cells using ABI1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Immunofluorescence analysis of ABI1 Antibody with paraffin-embedded human brain tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.
Application
Western blot analysis of ABI1 Antibody in MCF-7, CEM, Jurkat cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
