Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 μg/mL.| Product Name | ABIN3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ABIN3 Antibody: LIND, ABIN-3, ABIN3, LIND, TNFAIP3-interacting protein 3, A20-binding inhibitor of NF-kappa-B activation 3 |
| Product description | ABIN3 Antibody: The nuclear factor NF-κB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-κB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-κB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-κB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-κB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-κB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.1) Verstrepen L, Carpentier I, Verhelst K, et al. Abins: A20 binding inhibitors of NFkappaB and apoptosis signaling. Biochem. Pharmacol.2009; 78:105-14. |
| Immunogen | ABIN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ABIN3. The immunogen is located within the last 50 amino acids of ABIN3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ABIN3 antibody can be used for detection of ABIN3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNIP3 |
|---|---|
| Alternative Names | ABIN3 Antibody: LIND ABIN-3 ABIN3 LIND TNFAIP3-interacting protein 3 A20-binding inhibitor of NF-kappa-B activation 3 |
Application
Western blot analysis of ABIN3 in human spleen tissue lysate with ABIN3 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunohistochemistry of ABIN3 in mouse spleen tissue with ABIN3 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | ABIN3 antibody can be used for detection of ABIN3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ABIN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ABIN3 Antibody is supplied in PBS containing 0.02% sodium azide. |
