Application
Western blot analysis of extracts from COS cells treated with EGF using ABL1 (Phospho-Thr735) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | ABL1 (phospho Thr735) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Abelson murine leukemia viral oncogene 1, ABL1, c-ABL, JTK7, p150 |
| Product description | The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1.1) Fainstein E., Oncogene 4:1477-1481(1989). |
| Immunogen | ABL1 (phospho Thr735) antibody was raised against a peptide sequence around phosphorylation site of threonine735 (S-V-T (p)-L-P) derived from Human ABL1. |
| Modification | p-Thr735 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ABL1 |
|---|---|
| Alternative Names | Abelson murine leukemia viral oncogene 1 ABL1 c-ABL JTK7 p150 |
| Molecular Weight(MW) | 135 kDa |
| Tissue Specificity | The antibody detects endogenous levels of ABL1 only when phosphorylated at threonine 735. |
| Entrez Gene | 25 |
|---|---|
| Protein Accession | NP_005148.2 |
Application
Western blot analysis of extracts from COS cells treated with EGF using ABL1 (Phospho-Thr735) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
