Application
Immunohistochemistry of ACE2 in human kidney tissue with ACE2 antibody at 2 μg/mL.Application
Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody (IN2) at 2 μg/mL.Application
Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 2 μg/mL.| Product Name | ACE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ACE2 Antibody: ACEH, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH |
| Product description | ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.1) Donoghue M, Hsieh F, Baronas E, et al. A novel angiotensin-converting enzyme-related carboxypeptidase (ACE2) converts angiotension I to angiotension 1-9. Circ. Res. 2000;87:1-9. |
| Immunogen | ACE2 antibody was raised against a synthetic peptide corresponding to amino acids near the center of human ACE2. The immunogen is located within amino acids 150 - 200 of ACE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/mL. A 90 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACE2 |
|---|---|
| Alternative Names | ACE2 Antibody: ACEH Angiotensin-converting enzyme 2 ACE-related carboxypeptidase ACEH |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | Anti-ACE2 has no cross response to ACE1. |
Application
Immunohistochemistry of ACE2 in human kidney tissue with ACE2 antibody at 2 μg/mL.
Application
Western blot analysis of ACE2 in human kidney lysate with ACE2 antibody (IN2) at 2 μg/mL.
Application
Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | ACE2 antibody can be used for the detection of ACE2 by Western blot at 0.5 to 2 μg/mL. A 90 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
