Application
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.Application
Immunocytochemistry staining of K562 cells using Acinus antibody at 10 μg/mL.Application
Immunofluorescence of Acinus in Hela cells with Acinus antibody at 10 μg/mL.Application
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (CP) at 1 μg/mL.| Product Name | Acinus Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acinus Antibody: ACN, ACINUS, fSAP152, KIAA0670, Apoptotic chromatin condensation inducer in the nucleus, Acinus |
| Product description | Acinus Antibody: Chromatin condensation and nuclear fragmentation (CCNF) is the hallmark of apoptosis. CCNF is triggered by the activation of members of caspase family, caspase activated DNase (CAD/DFF40), and several novel proteins including AIF and CIDE. A new inducer of chromatin condensation was recently identified and designated Acinus (for apoptotic chromatin condensation inducer in the nucleus). Acinus is cleaved by caspase-3 and an additional unknown protease generating a small active peptide p17, which causes chromatin condensation in vitro when it is added to purified nuclei. Acinus also induces apoptotic chromatin condensation in cells. Acinus is ubiquitously expressed. Three different spliced forms of Acinus have been identified in human and mouse and designated AcinusL, AcinusS and AcinusS'.1) Zamzami N, Kroemer G. Condensed matter in cell death. Nature 1999 ;401:127-8. |
| Immunogen | Acinus antibody was raised against a peptide corresponding to amino acids near the C-terminus of the cleaved active peptide p17, which are identical to those of mouse Acinus. The immunogen is located within amino acids 1050 - 1100 of Acinus. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| Acinus antibody can be used for detection of Acinus by Western blot at 1 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACIN1 |
|---|---|
| Alternative Names | Acinus Antibody: ACN ACINUS fSAP152 KIAA0670 Apoptotic chromatin condensation inducer in the nucleus Acinus |
| Molecular Weight(MW) | 220 kDa |
| Sequence Similarities | 3) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunofluorescence of Acinus in K562 cells with Acinus antibody at 20 μg/ml.
Application
Immunocytochemistry staining of K562 cells using Acinus antibody at 10 μg/mL.
Application
Immunofluorescence of Acinus in Hela cells with Acinus antibody at 10 μg/mL.
Application
Western blot analysis of Acinus in K562 whole cell lysate with Acinus antibody (CP) at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | Acinus antibody can be used for detection of Acinus by Western blot at 1 μg/mL. An approximate 220 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Acinus antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Acinus Antibody is supplied in PBS containing 0.02% sodium azide. |
