Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).| Product Name | ACK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1 |
| Product description | This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.1) Mahajan, K., et al. J. Cell. Physiol. 224(2):327-333(2010) |
| Immunogen | This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-290 amino acids from the Central region of human ACK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNK2 |
|---|---|
| Alternative Names | Activated CDC42 kinase 1 ACK-1 Tyrosine kinase non-receptor protein 2 TNK2 ACK1 |
| Molecular Weight(MW) | 115 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the ACK1 antibody detected the ACK1 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
