ACOT12 contains 2 acyl coenzyme A hydrolase domains and 1 START domain. It hydrolyzes acetyl-CoA to acetate and CoA.1) Hunt, M.C., (2006) FASEB J. 20 (11), 1855-1864.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACOT12.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ACOT12 antibody can be used for detection of ACOT12 by ELISA at 1:12500. ACOT12 antibody can be used for detection of ACOT12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application Notes
ACOT12 antibody can be used for detection of ACOT12 by ELISA at 1:12500. ACOT12 antibody can be used for detection of ACOT12 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ACOT12 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.