Application
Antibody used in IHC on Human THP-1 at: 1:200.Application
Antibody used in IHC on Human THP-1 at: 1:200.Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Product Name | ACSL3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ACSL3 is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity, subcellular localization, and tissue distribution, all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters, and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain, and preferentially utilizes myristate, arachidonate, and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene.1) Yao, H. (2008) J. Biol. Chem. 283 (2), 849-854. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ACSL3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ACSL3 antibody can be used for detection of ACSL3 by ELISA at 1:312500. ACSL3 antibody can be used for detection of ACSL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACSL3 |
|---|---|
| Alternative Names | ACSL3 ACS3 FACL3 PRO2194 |
| Molecular Weight(MW) | 80 kDa |
Application
Antibody used in IHC on Human THP-1 at: 1:200.
Application
Antibody used in IHC on Human THP-1 at: 1:200.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.| Application Notes | ACSL3 antibody can be used for detection of ACSL3 by ELISA at 1:312500. ACSL3 antibody can be used for detection of ACSL3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ACSL3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
