Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
ACTG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate cancinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ACTG2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 2, encoded by this gene, is a smooth muscle actin found in enteric tissues.1) Sun, Q., et al. J. Biol. Chem. 284(47):32582-32590(2009) |
| Immunogen | This ACTG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 343-371 amino acids from the C-terminal region of human ACTG2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTG2 |
|---|---|
| Alternative Names | Actin gamma-enteric smooth muscle Alpha-actin-3 Gamma-2-actin Smooth muscle gamma-actin ACTG2 ACTA3 ACTL3 ACTSG |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
ACTG2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate cancinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
