Application
Antibody used in IHC on rACTNX-GFP at: 1:1000.Application
Antibody used in WB on Human HEK293 at 1:1000.Application
Antibody used in WB on rACTNX-GFP at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in WB on Rat Cortical Neurons at 1:1000.| Product Name | Actin alpha 1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Li, Q., (2007) J. Neurochem. 103 (6), 2391-2400. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Actin alpha 1 antibody can be used for detection of Actin alpha 1 by ELISA at 1:312500. Actin alpha 1 antibody can be used for detection of Actin alpha 1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN1 |
|---|---|
| Alternative Names | ACTN1 FLJ40884 BDPLT15 |
| Molecular Weight(MW) | 103 kDa |
Application
Antibody used in IHC on rACTNX-GFP at: 1:1000.
Application
Antibody used in WB on Human HEK293 at 1:1000.
Application
Antibody used in WB on rACTNX-GFP at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in WB on Rat Cortical Neurons at 1:1000.| Application Notes | Actin alpha 1 antibody can be used for detection of Actin alpha 1 by ELISA at 1:312500. Actin alpha 1 antibody can be used for detection of Actin alpha 1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store Actin alpha 1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
