Application
Antibody used in WB on Human heart at 1.25 ug/ml.Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.Application
Antibody used in WB on ACTNX-GFP transfected at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).| Product Name | Actin alpha 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The alpha-actinins are a multigene family of four actin-binding proteins related to dystrophin. The two skeletal muscle isoforms of alpha-actinin (ACTN2 and ACTN3) are major structural components of the Z-line involved in anchoring the actin-containing thin filaments. In humans, ACTN2 is expressed in all muscle fibres, while ACTN3 expression is restricted to a subset of type 2 fibres. Murine Actn2 and Actn3 are differentially expressed, spatially and temporally, during embryonic development and, in contrast to humans, alpha-actinin-2 expression does not completely overlap alpha-actinin-3 in postnatal skeletal muscle, suggesting independent function.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Transcript variants resulting from the use of multiple poly_A sites have been observed.1) Eldstrom, J., FEBS Lett. 547 (1-3), 205-211 (2003). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ACTN2 |
|---|---|
| Alternative Names | ACTN2 CMD1AA |
| Molecular Weight(MW) | 98 kDa |
Application
Antibody used in WB on Human heart at 1.25 ug/ml.
Application
Antibody used in IHC on Human Muscle at 4.0-8.0 ug/ml.
Application
Antibody used in WB on ACTNX-GFP transfected at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).| Application Notes | Actin alpha 2 antibody can be used for detection of Actin alpha 2 by ELISA at 1:312500. Actin alpha 2 antibody can be used for detection of Actin alpha 2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
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| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store Actin alpha 2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
