Application
Antibody used in WB on ACTNX-GFP transfected at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).Application
Antibody used in WB on Human HCT116 at 0.2-1 ug/ml.| Product Name | Actin alpha 4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha actinins belong to the spectrin superfamily which is a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. ACTN4 is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in its gene have been associated with focal and segmental glomerulosclerosis.Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and thought to be involved in metastatic processes. Mutations in this gene have been associated with focal and segmental glomerulosclerosis.1) Kimura, K., (2006) Genome Res. 16 (1), 55-65. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human Actin alpha 4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Actin alpha 4 antibody can be used for detection of Actin alpha 4 by ELISA at 1:62500. Actin alpha 4 antibody can be used for detection of Actin alpha 4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN4 |
|---|---|
| Alternative Names | ACTN4 DKFZp686K23158 FSGS FSGS1 ACTININ-4 |
| Molecular Weight(MW) | 105 kDa |
Application
Antibody used in WB on ACTNX-GFP transfected at: 1:1000 (Lane1: 10 ug ACTN1-GFP transfected COS-7 lysate, Lane2: 10 ug ACTN2-GFP transfected COS-7 lysate, Lane3: 10 ug ACTN3-GFP transfected COS-7 lysate, Lane4: 10 ug ACTN4-GFP transfected COS-7 lysate).
Application
Antibody used in WB on Human HCT116 at 0.2-1 ug/ml.| Application Notes | Actin alpha 4 antibody can be used for detection of Actin alpha 4 by ELISA at 1:62500. Actin alpha 4 antibody can be used for detection of Actin alpha 4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store Actin alpha 4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
