Application
Western blot analysis of ACTB/ACTC Antibody in mouse testis tissue lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | Actin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. ACTB/ACTC are nonmuscle cytoskeletal actins and major constituents of the contractile apparatus. Defects in ACTB are a cause of juvenile-onset dystonia. Defects in ACTC have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC).1) Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007) Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007) Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007) |
| Immunogen | This Actin (ACTB/ACTC) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 38-67 amino acids from the N-terminal region of human Actin (ACTB/ACTC). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | ACTC1 |
|---|---|
| Alternative Names | Actin alpha cardiac muscle 1 Alpha-cardiac actin ACTC1 ACTC |
| Molecular Weight(MW) | 42 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: C. Elegans |
Application
Western blot analysis of ACTB/ACTC Antibody in mouse testis tissue lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ACTB/ACTC antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Confocal immunofluorescent analysis of Actin (ACTB/ACTC) Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
