Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Product Name | ACTN2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles.1) Saus, E., et al.Psychiatr Res 44(14):971-978(2010) |
| Immunogen | This ACTN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 751-779 amino acids from the C-terminal region of human ACTN2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACTN2 |
|---|---|
| Alternative Names | Alpha-actinin-2 Alpha-actinin skeletal muscle isoform 2 F-actin cross-linking protein ACTN2 |
| Molecular Weight(MW) | 104 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
