Application
Western blot analysis of anti-ACTN4 Antibody in Hela cell line lysates (35ug/lane)| Product Name | ACTN4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This protein is a nonmuscle, alpha actinin isoform which is concentrated in the cytoplasm, and is thought to be involved in metastatic processes. Mutations in the gene encoding this protein have been associated with focal and segmental glomerulosclerosis.1) Kikuchi,S., Clin. Cancer Res. 14 (17), 5348-5356 (2008) |
| Immunogen | This ACTN4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 192-220 amino acids from the N-terminal region of human ACTN4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | ACTN4 |
|---|---|
| Alternative Names | Alpha-actinin-4 F-actin cross-linking protein Non-muscle alpha-actinin 4 ACTN4 |
| Molecular Weight(MW) | 105 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis of anti-ACTN4 Antibody in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
