Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ADA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine deaminase, Adenosine aminohydrolase, ADA, ADA1 |
| Product description | This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This ADA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 287-314 amino acids from the C-terminal region of human ADA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADA |
|---|---|
| Alternative Names | Adenosine deaminase Adenosine aminohydrolase ADA ADA1 |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis in HL-60 cell line lysates (35ug/lane).
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
