Application
Western blot analysis of anti-ADAMTS13 Antibody in CEM cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADAMTS13 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ADAMTS13 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | A disintegrin and metalloproteinase with thrombospondin motifs 13, ADAM-TS 13, ADAM-TS13, ADAMTS-13, von Willebrand factor-cleaving protease, vWF-CP, vWF-cleaving protease, ADAMTS13, C9orf8 |
| Product description | ADAMTS13 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motif) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme is the von Willebrand Factor (vWF)-cleaving protease, which is responsible for cleaving at the site of Tyr842-Met843 of the vWF molecule. A deficiency of this enzyme is associated with thrombotic thrombocytopenic purpura.1) Zheng X., Chung D., Takayama T.K.J. Biol. Chem. 276:41059-41063(2001) |
| Immunogen | This ADAMTS13 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 829-858 amino acids from the Central region of human ADAMTS13. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAMTS13 |
|---|---|
| Alternative Names | A disintegrin and metalloproteinase with thrombospondin motifs 13 ADAM-TS 13 ADAM-TS13 ADAMTS-13 von Willebrand factor-cleaving protease vWF-CP vWF-cleaving protease ADAMTS13 C9orf8 |
| Molecular Weight(MW) | 154 kDa |
Application
Western blot analysis of anti-ADAMTS13 Antibody in CEM cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with ADAMTS13 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
