Application
Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.| Product Name | ADAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ADAP Antibody: ADAP, PRO0823, SLAP-130, SLAP130, FYN-binding protein, Adhesion and degranulation promoting adaptor protein, ADAP |
| Product description | ADAP Antibody: The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-κB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.1) Griffiths EK, Krawczyk C, Kong YY, et al. Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap. Science2001; 293:2260-3. |
| Immunogen | ADAP antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human ADAP. The immunogen is located within amino acids 640 - 690 of ADAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FYB |
|---|---|
| Alternative Names | ADAP Antibody: ADAP PRO0823 SLAP-130 SLAP130 FYN-binding protein Adhesion and degranulation promoting adaptor protein ADAP |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
Application
Western blot analysis of ADAP in K562 cell lysate with ADAP antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunocytochemistry of ADAP in K562 cells with ADAP antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ADAP antibody can be used for detection of ADAP by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ADAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ADAP Antibody is supplied in PBS containing 0.02% sodium azide. |
