Application
Western blot analysis of lysed extracts from 293 and HeLa cells using ADD1 (Ab-726).Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Ab-726). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ADD1 (Ab-726).| Product Name | ADD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Adducins are a family of cytoskeleton proteins encoded by three genes (alpha, beta, gamma). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca(2+)/calmodulin and is a substrate for protein kinases A and C. Alternative splicing results in multiple variants encoding distinct isoforms; however, not all variants have been fully described.1) Pariser H, et al. Proc Natl Acad Sci USA 2005 Aug 30; 102 (35): 12407-12Tamaru S, et al. Biochem Biophys Res Commun 2005 Jul 01; 332 (2): 347-51Barkalow KL, et al. J Cell Biol 2003 May 12; 161 (3): 557-70 |
| Immunogen | ADD1 (Ab-726) antibody was raised against a peptide sequence around aa.724~728 (T-P-S-F-L) derived from Human ADD1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADD1 |
|---|---|
| Alternative Names | ADDA ERBB ERBB1 HER1 ADDA Erythrocyte adducin alpha subunit |
| Molecular Weight(MW) | 130 kDa |
| Tissue Specificity | This antibody detects endogenous level of total ADD1 protein. |
| Entrez Gene | 118 |
|---|---|
| Protein Accession | NP_001110.2 |
Application
Western blot analysis of lysed extracts from 293 and HeLa cells using ADD1 (Ab-726).
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ADD1 (Ab-726). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using ADD1 (Ab-726).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
