Application
Western blot analysis of anti-ADK Pab in mouse liver tissue lysate (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ADK antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ADK Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Adenosine kinase, AK, Adenosine 5'-phosphotransferase, ADK |
| Product description | Adenosine kinase is an abundant enzyme in mammalian tissues. The enzyme catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Alternative splicing results in two transcript variants encoding different isoforms. Both isoforms of the enzyme phosphorylate adenosine with identical kinetics and both require Mg2+ for activity.1) Szkotak, A.J., et al., J. Membr. Biol. 192(3):169-179 (2003). Singh, B., et al., DNA Cell Biol. 20(1):53-65 (2001). Van Rompay, A.R., et al., Eur. J. Biochem. 261(2):509-517 (1999). Mathews, I.I., et al., Biochemistry 37(45):15607-15620 (1998). McNally, T., et al., Biochem. Biophys. Res. Commun. 231(3):645-650 (1997). |
| Immunogen | This ADK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-352 amino acids from the C-terminal region of human ADK. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | ADK |
|---|---|
| Alternative Names | Adenosine kinase AK Adenosine 5'-phosphotransferase ADK |
| Molecular Weight(MW) | 41 kDa |
Application
Western blot analysis of anti-ADK Pab in mouse liver tissue lysate (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ADK antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
