Application
Western blot analysis of ADRA1B Antibody in NCI-H460 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ADRA1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-1B adrenergic receptor, Alpha-1B adrenoreceptor, Alpha-1B adrenoceptor, ADRA1B |
| Product description | Alpha-1-adrenergic receptors (alpha-1-ARs) are members of the G protein-coupled receptor superfamily. They activate mitogenic responses and regulate growth and proliferation of many cells. There are 3 alpha-1-AR subtypes: alpha-1A, -1B and -1D, all of which signal through the Gq/11 family of G-proteins and different subtypes show different patterns of activation. This protein encodes alpha-1B-adrenergic receptor, which induces neoplastic transformation when transfected into NIH 3T3 fibroblasts and other cell lines. Thus, this normal cellular gene is identified as a protooncogene. This protein comprises 2 exons and a single large intron of at least 20 kb that interrupts the coding region.1) Mathias,R.A., J. Allergy Clin. Immunol. 125 (2), 336-346 (2010) |
| Immunogen | This ADRA1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-297 amino acids from the Central region of human ADRA1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADRA1B |
|---|---|
| Alternative Names | Alpha-1B adrenergic receptor Alpha-1B adrenoreceptor Alpha-1B adrenoceptor ADRA1B |
| Molecular Weight(MW) | 57 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of ADRA1B Antibody in NCI-H460 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human brain with ADRA1B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
