Application
Western blot analysis of ALKBH3 Antibody in mouse kidney tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ALKBH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, 11411-, Alkylated DNA repair protein alkB homolog 3, DEPC-1, Prostate cancer antigen 1, ALKBH3, ABH3, DEPC1 |
| Product description | The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.1) Sundheim,O., et.al., EMBO J. 25 (14), 3389-3397 (2006) |
| Immunogen | This ALKBH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 242-269 amino acids from the C-terminal region of human ALKBH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ALKBH3 |
|---|---|
| Alternative Names | Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3 11411- Alkylated DNA repair protein alkB homolog 3 DEPC-1 Prostate cancer antigen 1 ALKBH3 ABH3 DEPC1 |
| Molecular Weight(MW) | 33 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of ALKBH3 Antibody in mouse kidney tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with ALKBH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
